GSM5573393: Astrocytes CTCF Untreated rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Neural
Cell type
Fetal astrocytes
NA
NA
Attributes by original data submitter
Sample
source_name
fetal human astrocytes
cell type
fetal human astrocytes
chip antibody
CTCF (#3418, Cell Signaling)
treatment
Untreated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S, NEB). ChIP-Seq with Drosophila spike-in chromatin (Cat. 53083, Active Motif) and Drosophila -specific histone variant, H2Av (Cat. 61686, Active Motif) were added to each sample prior IP for normalization.